Generation and Starch Characterization of Non-Transgenic BEI and BEIIb Double Mutant Rice (Oryza sativa) with Ultra-High Level of Resistant Starch

Abstract Background Cereals high in resistant starch (RS) are gaining popularity, as their intake is thought to help manage diabetes and prediabetes. Number of patients suffering from also increasing Asian countries where people consume rice a staple food, hence generation practically growable RS line has been anticipated. It known that suppression branching enzyme (BE) IIb increases content cereals. To further increase for more practical use, we generated non-transgenic be1 be2b double mutant ( Oryza sativa ) line, which completely lacked both proteins, by crossing with mutant. Results The showed decrease intermediate amylopectin chains an long compared . amylose (51.7%) was the highest among all pre-existing lines. understand effects chewing cooked cooking flour on content, mashed un-mashed well raw gelatinized were measured using its parent contents (28.4% 35.1%, respectively) 3-fold higher than those Gel-filtration analyses treated digestive enzymes composed degradation products chains. Seed weight approximately 60% wild type rather heavier Conclusions endosperm enriched This led great grains single this study must serve good material ultra-high cultivar.